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AAT Bioquest quest graphtm ec 50 calculator
Quest Graphtm Ec 50 Calculator, supplied by AAT Bioquest, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews

quest graphtm ec 50 calculator - by Bioz Stars, 2026-10

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Article Title: Sideritis scardica Extracts Demonstrate Neuroprotective Activity against Aβ 25–35 Toxicity
Article Snippet: The EC 50 values were estimated online with the use of an EC 50 calculator made by AAT Bioquest [ ].

Article Title: Effect of a Low-Molecular-Weight Allosteric Agonist of the Thyroid-Stimulating Hormone Receptor on Basal and Thyroliberin-Stimulated Activity of Thyroid System in Diabetic Rats
Article Snippet: The EC 50 value for the AC-stimulating effects of TPY3m was calculated using the algorithm presented at https://www.aatbio.com/tools/ec50-calculator , accessed on 17 September 2024.

Article Title: Screening bisphenols in complex samples via a planar Arxula adeninivorans bioluminescence bioassay
Article Snippet: Using the Hill function, three-parameter calibration curves were determined, and the EC 50 values were calculated with the freeware EC 50 Calculator (AAT Bioquest, Sunnyvale, CA, USA).

Article Title: Synthesis and Biological Evaluation of Dantrolene‐Like Hydrazide and Hydrazone Analogues as Multitarget Agents for Neurodegenerative Diseases
Article Snippet: EC 50 values were calculated using AATBioquest EC 50 calculator.

Article Title: Deimmunization of flagellin for repeated administration as a vaccine adjuvant
Article Snippet: EC 50 was calculated using triplicate OD 620 nm values for each protein concentration over a wide range of protein concentrations (0.03719 nM to 76.16 nM at the AAT Bioquest website [ https://www.aatbio.com/tools/ec50-calculator ]).

Concentration Assay:

Article Title: Comparison of Chemical Composition and Biological Activities of Eight Selaginella Species
Article Snippet: The ability of samples to absorb the generated radicals was monitored by measuring the fluorescence (excitation/emission 485/535 nm), recorded at 5 min intervals for 2 h using a SpectraMax i3x microplate reader (Molecular Devices, San Jose, CA, USA). .. The EC 50 values were determined according to the concentration range using AAT Bioquest EC 50 calculator [ ]. ..

Article Title: Headspace/GC–MS Analysis and Investigation of Antibacterial, Antioxidant and Cytotoxic Activity of Essential Oils and Hydrolates from Rosmarinus officinalis L. and Lavandula angustifolia Miller
Article Snippet: DMSO was used to solubilize the formazan crystals and the absorbance was read by Tecan SunriseTM (Tecan Group Ltd., Männedorf, Switzerland) UV-vis spectrophotometer at 595 nm. .. The obtained optical density values were converted into percentage of cell viability and the data were elaborated with AAT Bioquest EC 50 Calculator (Sunnyvale, CA, USA) [ ] in order to obtain the concentration at which the investigated substance exerts half of its maximal response values (EC 50 ). ..

Activity Assay:

Article Title: In vitro and in vivo efficacy of thiacloprid against Echinococcus multilocularis
Article Snippet: PGI measurements were performed as described earlier [ ], except that an Infinite M200 PRO reader (Tecan, Männedorf, Switzerland) was used to measure the increase in absorbance at 340 nm. .. PGI activity was calculated using the EC 50 calculator ( https://www.aatbio.com/tools/ec50-calculator ) from the linear regression of the enzyme reaction over time and presented as a percentage relative to the values obtained by treatment of vesicles with 0.1% Triton X-100. ..

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Network pharmacology and targets analysis of baicalein against PRRSV . A Interaction analysis of target genes between baicalein and PRRSV-related. B PPI network analysis. 90 potential targets were input into a String database, and a PPI network was constructed ( P -value < 1.0 e−16 ). C Topological analysis by Cytoscape 3.10.0. The seven core targets were screened and highlighted in the right box. The core targets screening criteria were set as degree > 31. D GO enrichment analysis of the 90 potential targets. E Top 10 of KEGG signalling pathway analysis of the 90 potential targets. The size of the dot represents the counts of proteins in this pathway; the colour of the dot corresponds to different P -value ranges.

Journal: Veterinary Research

Article Title: Baicalein inhibits PRRSV through direct binding, targeting EGFR, and enhancing immune response

doi: 10.1186/s13567-024-01440-5

Figure Lengend Snippet: Network pharmacology and targets analysis of baicalein against PRRSV . A Interaction analysis of target genes between baicalein and PRRSV-related. B PPI network analysis. 90 potential targets were input into a String database, and a PPI network was constructed ( P -value < 1.0 e−16 ). C Topological analysis by Cytoscape 3.10.0. The seven core targets were screened and highlighted in the right box. The core targets screening criteria were set as degree > 31. D GO enrichment analysis of the 90 potential targets. E Top 10 of KEGG signalling pathway analysis of the 90 potential targets. The size of the dot represents the counts of proteins in this pathway; the colour of the dot corresponds to different P -value ranges.

Article Snippet: G EC 50 of baicalein against PRRSV was constructed by GraphPad prism 8.0.

Techniques: Construct

Binding of baicalein with potential targets

Journal: Veterinary Research

Article Title: Baicalein inhibits PRRSV through direct binding, targeting EGFR, and enhancing immune response

doi: 10.1186/s13567-024-01440-5

Figure Lengend Snippet: Binding of baicalein with potential targets

Article Snippet: G EC 50 of baicalein against PRRSV was constructed by GraphPad prism 8.0.

Techniques: Binding Assay

Molecular docking and MD stimulation of baicalein with EGFR . A Active pocket of EGFR prediction. The volume of the EGFR binding pocket was calculated with CHECOM algorithm by Yinfo technology. Active pocket of EGFR was shown in red with 25,465 Å 3 . B Molecular docking of EGFR (Uniprot ID: A0A5G2QBP1) with baicalein (Compound CID: 5281605) in a 3D model and 2D ligand interaction diagram. C Root Mean Square Deviation (RMSD) of baicalein and EGFR during molecular dynamics simulation. D Hydrogen bond numbers between baicalein and EGFR at different times. E Molecular docking of baicalein with another six core protein targets. E1-E6: SRC, TP53, MMP9, STAT3, INS, and ESR1.

Journal: Veterinary Research

Article Title: Baicalein inhibits PRRSV through direct binding, targeting EGFR, and enhancing immune response

doi: 10.1186/s13567-024-01440-5

Figure Lengend Snippet: Molecular docking and MD stimulation of baicalein with EGFR . A Active pocket of EGFR prediction. The volume of the EGFR binding pocket was calculated with CHECOM algorithm by Yinfo technology. Active pocket of EGFR was shown in red with 25,465 Å 3 . B Molecular docking of EGFR (Uniprot ID: A0A5G2QBP1) with baicalein (Compound CID: 5281605) in a 3D model and 2D ligand interaction diagram. C Root Mean Square Deviation (RMSD) of baicalein and EGFR during molecular dynamics simulation. D Hydrogen bond numbers between baicalein and EGFR at different times. E Molecular docking of baicalein with another six core protein targets. E1-E6: SRC, TP53, MMP9, STAT3, INS, and ESR1.

Article Snippet: G EC 50 of baicalein against PRRSV was constructed by GraphPad prism 8.0.

Techniques: Binding Assay

Antiviral effect of baicalein on PRRSV infection in vitro . A 3D chemical structure of baicalein. B 50% cytotoxic concentration (CC 50 ) analysis. C Copies number of 10 –1 , 10 –2 , 10 – 3 fold dilution PRRSV. D CPEs of MARC-145 cells. MARC-145 cells were treated with DMSO, baicalein (80 μg/mL), and ribavirin (120 μM), respectively, and infected with PRRSV (100 TCID 50 ). PBS was used as a mock infection in the negative group. Scale bar = 100 μm. E The antiviral effect of baicalein on PRRSV was examined through the calculation of viral copies at various concentration gradients. F Immunofluorescence assay of protein N. Scale bar = 100 μm. G EC 50 of baicalein against PRRSV was constructed by GraphPad prism 8.0. The data are expressed as mean ± SD. Each experiment was performed with three biological replicates and three technical replicates. H Mean fluorescence intensity (Mean ± SEM, n = 3). * P < 0.05; ** P < 0.01, *** P < 0.005, **** P < 0.0001 compared with the DMSO treatment group.

Journal: Veterinary Research

Article Title: Baicalein inhibits PRRSV through direct binding, targeting EGFR, and enhancing immune response

doi: 10.1186/s13567-024-01440-5

Figure Lengend Snippet: Antiviral effect of baicalein on PRRSV infection in vitro . A 3D chemical structure of baicalein. B 50% cytotoxic concentration (CC 50 ) analysis. C Copies number of 10 –1 , 10 –2 , 10 – 3 fold dilution PRRSV. D CPEs of MARC-145 cells. MARC-145 cells were treated with DMSO, baicalein (80 μg/mL), and ribavirin (120 μM), respectively, and infected with PRRSV (100 TCID 50 ). PBS was used as a mock infection in the negative group. Scale bar = 100 μm. E The antiviral effect of baicalein on PRRSV was examined through the calculation of viral copies at various concentration gradients. F Immunofluorescence assay of protein N. Scale bar = 100 μm. G EC 50 of baicalein against PRRSV was constructed by GraphPad prism 8.0. The data are expressed as mean ± SD. Each experiment was performed with three biological replicates and three technical replicates. H Mean fluorescence intensity (Mean ± SEM, n = 3). * P < 0.05; ** P < 0.01, *** P < 0.005, **** P < 0.0001 compared with the DMSO treatment group.

Article Snippet: G EC 50 of baicalein against PRRSV was constructed by GraphPad prism 8.0.

Techniques: Infection, In Vitro, Concentration Assay, Immunofluorescence, Construct, Fluorescence

Baicalein inhibited PRRSV proliferation in pre-, co-, and post-treatment modes in MARC-145 cells . A Schematic diagram of time-of-addition assay. Briefly, MARC-145 cells were infected with PRRSV (100 TCID 50 ), and the cells were treated with 80 μg/mL baicalein for different times before (pre-treatment), during (co-treatment) or post-infection (post-treatment), as shown in the schedules. At 48 hpi, the cells and supernatants were harvested for analysis. B PRRSV copy number calculation using absolute quantification. C PRRSV-ORF7 relative expression analysis using qRT-PCR. D Virus titres of PRRSV. TCID 50 of PRRSV was calculated by the Spearman-Kärber method. Each experiment was performed with three biological replicates and three technical replicates. The data are expressed as mean ± SD. *, P < 0.05; **, P < 0.01, ***, P < 0.005, ****, P < 0.0001 compared with the DMSO treatment group.

Journal: Veterinary Research

Article Title: Baicalein inhibits PRRSV through direct binding, targeting EGFR, and enhancing immune response

doi: 10.1186/s13567-024-01440-5

Figure Lengend Snippet: Baicalein inhibited PRRSV proliferation in pre-, co-, and post-treatment modes in MARC-145 cells . A Schematic diagram of time-of-addition assay. Briefly, MARC-145 cells were infected with PRRSV (100 TCID 50 ), and the cells were treated with 80 μg/mL baicalein for different times before (pre-treatment), during (co-treatment) or post-infection (post-treatment), as shown in the schedules. At 48 hpi, the cells and supernatants were harvested for analysis. B PRRSV copy number calculation using absolute quantification. C PRRSV-ORF7 relative expression analysis using qRT-PCR. D Virus titres of PRRSV. TCID 50 of PRRSV was calculated by the Spearman-Kärber method. Each experiment was performed with three biological replicates and three technical replicates. The data are expressed as mean ± SD. *, P < 0.05; **, P < 0.01, ***, P < 0.005, ****, P < 0.0001 compared with the DMSO treatment group.

Article Snippet: G EC 50 of baicalein against PRRSV was constructed by GraphPad prism 8.0.

Techniques: Infection, Quantitative Proteomics, Expressing, Quantitative RT-PCR, Virus

Baicalein directly interacted with PRRSV . A Schematic diagram of experimental design. Briefly, PRRSV (100 TCID 50 ) was subjected to incubation with baicalein at different concentrations (20, 40, and 80 μg/mL) in essential medium for a duration of 1 h at 37 °C. Following this incubation, PRRSV was isolated from baicalein through the process of ultrafiltration. The retrieved PRRSV was then challenged to MARC-145 cells. Subsequently, at 48 hpi, the cells were collected for analysis. B PRRSV copy number calculation using absolute quantification. C PRRSV-ORF7 relative expression analysis using qRT-PCR. D Virus titres of PRRSV. TCID 50 of PRRSV were calculated by the Spearman-Kärber method. Each experiment was performed with three biological replicates and three technical replicates. The data are expressed as mean ± SD. * P < 0.05; ** P < 0.01, *** P < 0.005, **** P < 0.0001 compared with the only PRRSV treatment group. E Active pocket of Gp5/M prediction. The volume of the Gp5/M binding pocket was calculated with CHECOM algorithm by Yinfo technology. Active pocket of Gp5/M was shown in green. F Molecular docking of Gp5/M (PRRSV-2 VR 2332, model 1, alphafold2) with baicalein (Compound CID: 5281605) in a 3D model. G Root Mean Square Deviation (RMSD) of baicalein and Gp5/M during MD simulation. H Hydrogen bond numbers between baicalein and Gp5/M in 100 ns.

Journal: Veterinary Research

Article Title: Baicalein inhibits PRRSV through direct binding, targeting EGFR, and enhancing immune response

doi: 10.1186/s13567-024-01440-5

Figure Lengend Snippet: Baicalein directly interacted with PRRSV . A Schematic diagram of experimental design. Briefly, PRRSV (100 TCID 50 ) was subjected to incubation with baicalein at different concentrations (20, 40, and 80 μg/mL) in essential medium for a duration of 1 h at 37 °C. Following this incubation, PRRSV was isolated from baicalein through the process of ultrafiltration. The retrieved PRRSV was then challenged to MARC-145 cells. Subsequently, at 48 hpi, the cells were collected for analysis. B PRRSV copy number calculation using absolute quantification. C PRRSV-ORF7 relative expression analysis using qRT-PCR. D Virus titres of PRRSV. TCID 50 of PRRSV were calculated by the Spearman-Kärber method. Each experiment was performed with three biological replicates and three technical replicates. The data are expressed as mean ± SD. * P < 0.05; ** P < 0.01, *** P < 0.005, **** P < 0.0001 compared with the only PRRSV treatment group. E Active pocket of Gp5/M prediction. The volume of the Gp5/M binding pocket was calculated with CHECOM algorithm by Yinfo technology. Active pocket of Gp5/M was shown in green. F Molecular docking of Gp5/M (PRRSV-2 VR 2332, model 1, alphafold2) with baicalein (Compound CID: 5281605) in a 3D model. G Root Mean Square Deviation (RMSD) of baicalein and Gp5/M during MD simulation. H Hydrogen bond numbers between baicalein and Gp5/M in 100 ns.

Article Snippet: G EC 50 of baicalein against PRRSV was constructed by GraphPad prism 8.0.

Techniques: Incubation, Isolation, Quantitative Proteomics, Expressing, Quantitative RT-PCR, Virus, Binding Assay

Baicalein improved growth performance and immunity of PRRSV-infected weaned piglets . A Schematic diagram of experimental design of baicalein inhibiting PRRSV infection in piglets. B Average daily gain (ADG) of PRRSV-infected piglets with baicalein treatment. C Antibody level of PRRSV-infected piglets with baicalein treatment. D - E Macroscopic lung lesion changes in PRRSV-infected piglets with baicalein treatment. Macroscopic lungs of piglets in each group were scored by a scoring system. F The lungs of piglets were collected, and sections were prepared to stain with haematoxylin and eosin (H&E). Black arrows indicate the histopathological alterations, including progressive loss of lung architecture, thickening of the interlobular septa, and infiltration of lymphocytes (Mean ± SEM, n = 3, scale bar = 100 μm).

Journal: Veterinary Research

Article Title: Baicalein inhibits PRRSV through direct binding, targeting EGFR, and enhancing immune response

doi: 10.1186/s13567-024-01440-5

Figure Lengend Snippet: Baicalein improved growth performance and immunity of PRRSV-infected weaned piglets . A Schematic diagram of experimental design of baicalein inhibiting PRRSV infection in piglets. B Average daily gain (ADG) of PRRSV-infected piglets with baicalein treatment. C Antibody level of PRRSV-infected piglets with baicalein treatment. D - E Macroscopic lung lesion changes in PRRSV-infected piglets with baicalein treatment. Macroscopic lungs of piglets in each group were scored by a scoring system. F The lungs of piglets were collected, and sections were prepared to stain with haematoxylin and eosin (H&E). Black arrows indicate the histopathological alterations, including progressive loss of lung architecture, thickening of the interlobular septa, and infiltration of lymphocytes (Mean ± SEM, n = 3, scale bar = 100 μm).

Article Snippet: G EC 50 of baicalein against PRRSV was constructed by GraphPad prism 8.0.

Techniques: Infection, Staining

Baicalein improved antiviral activity of PRRSV-infected piglets . A - B IFA analysis of protein N using a rabbit PRRSV N protein polyclonal antibody (1:100, Bioss, Beijing, China). Expression of the protein N is indicated with red arrows. Scale bar = 100 μm; local enlarged images are shown in the right, scale bar = 20 μm (Mean ± SEM, n = 3). C - D Expression of the PRRSV N protein in lung tissues was detected using western blotting (Mean ± SEM, n = 3). E Viral loads of PRRSV calculated by absolute quantitation. F - G Expression of PRRSV protein N in lung tissues was detected by IHC with a rabbit PRRSV N protein polyclonal antibody (1:100, Bioss, Beijing, China). Expression of the N protein is indicated with black arrows. Scale bar = 100 μm (Mean ± SEM, n = 3). * P < 0.05; ** P < 0.01 compared with the PRRSV group.

Journal: Veterinary Research

Article Title: Baicalein inhibits PRRSV through direct binding, targeting EGFR, and enhancing immune response

doi: 10.1186/s13567-024-01440-5

Figure Lengend Snippet: Baicalein improved antiviral activity of PRRSV-infected piglets . A - B IFA analysis of protein N using a rabbit PRRSV N protein polyclonal antibody (1:100, Bioss, Beijing, China). Expression of the protein N is indicated with red arrows. Scale bar = 100 μm; local enlarged images are shown in the right, scale bar = 20 μm (Mean ± SEM, n = 3). C - D Expression of the PRRSV N protein in lung tissues was detected using western blotting (Mean ± SEM, n = 3). E Viral loads of PRRSV calculated by absolute quantitation. F - G Expression of PRRSV protein N in lung tissues was detected by IHC with a rabbit PRRSV N protein polyclonal antibody (1:100, Bioss, Beijing, China). Expression of the N protein is indicated with black arrows. Scale bar = 100 μm (Mean ± SEM, n = 3). * P < 0.05; ** P < 0.01 compared with the PRRSV group.

Article Snippet: G EC 50 of baicalein against PRRSV was constructed by GraphPad prism 8.0.

Techniques: Activity Assay, Infection, Expressing, Western Blot, Quantitation Assay

Baicalein improved anti-inflammatory and antioxidant activity of PRRSV-infected weaned piglets . A The relative expression of inflammatory cytokines in the lung. B The antioxidant capacity in serum, liver and lung. The data are expressed as mean ± SD ( n = 5). T-AOC Total antioxidant capacity, GSH-PX Glutathione peroxidase, MDA Malondialdehyde, CAT catalase, SOD Superoxide dismutase.

Journal: Veterinary Research

Article Title: Baicalein inhibits PRRSV through direct binding, targeting EGFR, and enhancing immune response

doi: 10.1186/s13567-024-01440-5

Figure Lengend Snippet: Baicalein improved anti-inflammatory and antioxidant activity of PRRSV-infected weaned piglets . A The relative expression of inflammatory cytokines in the lung. B The antioxidant capacity in serum, liver and lung. The data are expressed as mean ± SD ( n = 5). T-AOC Total antioxidant capacity, GSH-PX Glutathione peroxidase, MDA Malondialdehyde, CAT catalase, SOD Superoxide dismutase.

Article Snippet: G EC 50 of baicalein against PRRSV was constructed by GraphPad prism 8.0.

Techniques: Antioxidant Activity Assay, Infection, Expressing

Baicalein blocked PRRSV entry via the EGFR-PI3K-AKT pathway . A PRRSV copy number calculation using absolute quantification. B PRRSV-ORF7 relative expression analysis using qRT-PCR. C Virus titres of PRRSV. TCID 50 of PRRSV were calculated by the Spearman-Kärber method. Each experiment was performed with three biological replicates and three technical replicates. The data are expressed as mean ± SD. D Protein expression in the lung of PRRSV-infected piglets by western blotting. E Band intensities were subjected to semi-quantitative analysis employing ImageJ software, and statistical significance of the results was analysed utilizing GraphPad Prism software. β-actin served as loading control. F Phosphorylation EGFR expression in the lung of PRRSV-infected piglets by IHC. Strongly positive signals are presented with red arrows in PRRSV group and slightly positive signals are presented with black arrows in PRRSV + Bai group. Scale bar = 50 μm. G Area of p-EGFR expression (Mean ± SEM, n = 3). *, P < 0.05; **, P < 0.01, ***, P < 0.005, ****, P < 0.0001 compared with the only PRRSV treatment group.

Journal: Veterinary Research

Article Title: Baicalein inhibits PRRSV through direct binding, targeting EGFR, and enhancing immune response

doi: 10.1186/s13567-024-01440-5

Figure Lengend Snippet: Baicalein blocked PRRSV entry via the EGFR-PI3K-AKT pathway . A PRRSV copy number calculation using absolute quantification. B PRRSV-ORF7 relative expression analysis using qRT-PCR. C Virus titres of PRRSV. TCID 50 of PRRSV were calculated by the Spearman-Kärber method. Each experiment was performed with three biological replicates and three technical replicates. The data are expressed as mean ± SD. D Protein expression in the lung of PRRSV-infected piglets by western blotting. E Band intensities were subjected to semi-quantitative analysis employing ImageJ software, and statistical significance of the results was analysed utilizing GraphPad Prism software. β-actin served as loading control. F Phosphorylation EGFR expression in the lung of PRRSV-infected piglets by IHC. Strongly positive signals are presented with red arrows in PRRSV group and slightly positive signals are presented with black arrows in PRRSV + Bai group. Scale bar = 50 μm. G Area of p-EGFR expression (Mean ± SEM, n = 3). *, P < 0.05; **, P < 0.01, ***, P < 0.005, ****, P < 0.0001 compared with the only PRRSV treatment group.

Article Snippet: G EC 50 of baicalein against PRRSV was constructed by GraphPad prism 8.0.

Techniques: Quantitative Proteomics, Expressing, Quantitative RT-PCR, Virus, Infection, Western Blot, Software, Control, Phospho-proteomics

Scheme summarizing the inhibitory effect of baicalein on PRRSV infection . The underlying antiviral mechanisms of baicalein including binding to PRRSV structural protein to directly kill the virus, inhibiting EGFR-PI3K-AKT pathway to block PRRSV entry, and boosting host immunity to inhibit PRRSV infection. The figure was generated by BioRender with authorised consent for publication

Journal: Veterinary Research

Article Title: Baicalein inhibits PRRSV through direct binding, targeting EGFR, and enhancing immune response

doi: 10.1186/s13567-024-01440-5

Figure Lengend Snippet: Scheme summarizing the inhibitory effect of baicalein on PRRSV infection . The underlying antiviral mechanisms of baicalein including binding to PRRSV structural protein to directly kill the virus, inhibiting EGFR-PI3K-AKT pathway to block PRRSV entry, and boosting host immunity to inhibit PRRSV infection. The figure was generated by BioRender with authorised consent for publication

Article Snippet: G EC 50 of baicalein against PRRSV was constructed by GraphPad prism 8.0.

Techniques: Infection, Binding Assay, Virus, Blocking Assay, Generated

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